枳壳总黄酮提取工艺与含量测定研究

Extraction and content determination of total flavonoid from Fructus Aurantii

目的：研究枳壳总黄酮提取工艺与含量测定方法。方法：采用正交实验，筛选枳壳总黄酮最佳提取工艺条件，用紫外分光光度法测定总黄酮的含量。结果：枳壳总黄酮的最佳提取条件为：在温度70℃，pH3．0条件下，加15倍的Ca（OH）2饱和溶液，浸提2h／次，提取3次，以柚皮苷为对照测定总黄酮含量，柚皮苷线性范围为2．592-41．472μg／mL，r=0．9999。结论：所建方法快速、简便，可用于枳壳药材质量控制。

Objective： To study the extraction process and content determination of total flavonoids from Fructus Aurantii. Methods ：The optimum extraction conditions of total flavonoids from Fructus Aurantii were screened with orthogonal test and the content of total flavonoid with standard hesperidin was determined by UV spectrophotometry. Results i The best extraction conditions were as follow： adding 15 times amount of Ca（ OH）2 ,pH 3. 0 and at 70 ℃ for 3 times in all,2 hours each time. The linear range of naringin was 2. 592 41. 472μg/mL r-0. 999 9. Conclusion：The methods are simple ,fast and can be used to control the quality of Fructus Aurantii.