克糖特Ⅱ号降血糖作用的实验研究

Experimental studies on hypoglycemic effect of KetangteⅡ

目的:研究克糖特Ⅱ号对链脲霉素致糖尿病小鼠的降血糖作用并初步探讨其机制。方法:对小鼠腹腔注射链脲霉素(STZ)150 mg/kg建立糖尿病模型(DM),将建模成功的小鼠随机分为5组:格列本脲组(ig格列本脲50 mg/kg.d)、克糖特Ⅱ号高、中、低剂量(ig克糖特Ⅱ号分别相当于生药25 g/kg.d,12.5 g/kg.d,6.25 g/kg.d),糖尿病模型组(ig等体积生理盐水)和空白对照组(ig等体积生理盐水),连续给药15 d。并在相应时间采血测定空腹血糖(FBG)、胰岛素、血清肌酐(Cr)、尿酸(UA)、白蛋白(A lb)等指标。结果:克糖特Ⅱ号具有明显的降血糖作用,其作用与剂量呈正相关,并能提高胰岛素水平。同时能不同程度地降低血清Cr、UA(P<0.01),升高A lb(P<0.05)。结论:克糖特Ⅱ号能降低链脲霉素所致糖尿病小鼠的血糖,增加胰岛素的分泌,对糖尿病尤其并发症的治疗有潜在的应用价值。其降血糖作用机制可能与促进胰岛素分泌有关。

Objective ： To investigate the hypoglycemic effects and its mechanism of KTT Ⅱ on streptozotocin （STZ） -induced diabetic mice. Methods：Diabetic model mice is established by injecting 150 mg/kg of STZ. These mice were divided into five groups randomly： model group, glibenclamide group （50 mg/kg · d, ig）, high dose group of KTT Ⅱ （25 g/kg ·d,ig）, middle dose group of KTT （12.5 g/kg · d, ig） and low dose group of KTTⅡ （6.25 g/kg · d, ig） , while the control and model group were administrated with the same volume of physiological saline for 15 days. During the experiment, the fasting blood glucose （FBG）, insulin, creatinine （Cr） , uric acid （UA）, albumen （Alb） were measured at different time. Results： KTT Ⅱ could reduce the blood glucose concentration significantly in STZ-induced diabetic mice which was positively correlated with dosage-effect and time-effect. K Ⅱ could raise insulin level, and decrease Cr and UA levels in serum （P 〈 0.01 ） , increase Alb level in serum （P 〈 0.05 ）. Conclusion ：The results suggested that KTT Ⅱ could decrease blood glucose, improve insulin level in STZ-induced diabetic mice, indicating that KTT Ⅱ may have a potential value for the treatment of DM and DM complications. The mechanism of reducing blood glucose is probably associated with promoting insulin secretion.