头穴针刺对脑出血大鼠脑组织能量代谢的影响

Effect of Scalp-acupuncture on Energy Metabolism of Brain Tissue in Intracerebral Hemorrhage Rats

目的:观察大鼠脑出血后血肿周围组织线粒体琥珀酸脱氢酶(SDH)、钠-钾ATP酶(Na+-K+-ATPase)及乳酸(LA)的动态变化规律,探讨针刺作用机制。方法:80只雄性Wistar大鼠随机分为空白组、假手术组、模型组、针刺组,后3组又分为24、721、68 h 3个小组,每组/小组8例。采用胶原酶/肝素联合注入法(右尾核,2μl)制备脑出血模型,给以"百会"透"太阳"穴电针治疗(连续波,频率2 Hz,电流1 mA,30 min),梯度离心制备血肿周围脑组织线粒体悬液,比色法观测SDH、Na+-K+-ATPase活性和LA含量的变化。结果:与空白对照及假手术组比较,模型组脑组织SDH活性72 h开始、线粒体Na+-K+-ATPase活性24 h开始明显下降(P<0.05),LA水平24 h开始显著增高(P<0.05);3个指标的变化情况都有随时间延长而加重的趋势。与假手术组比,针刺组SDH(168 h开始)和线粒体Na+-K+-ATPase活性(24 h开始)也显著降低(P<0.05),但绝大多数时段明显高于模型组(P<0.05);针刺组LA水平各时段均显著低于模型组(P<0.05),但247、2 h显著高于假手术组(P<0.05)。结论:针刺可改善脑出血后的能量代谢,调节线粒体功能,减少乳酸蓄积。

Objective To observe the effect of electroacupuncture （EA） on mitochondrial SDH and Na^＋-K^＋-ATPase activity, and lactate acid （LA） content in the peri-hemotoma tissue in rats with intracerebral hemorrhage （ICH） so as to explore its mechanisms in resisting cerebral ischemia. Methods A total of 80 Wistar rats were randomized into normal group, sham group, model group, EA group. The last three groups were further divided into 24 h, 72 h and 168 h subgroups respectively, with 8 cases in each group/subgroups. ICH model was established by caudatum-injection （right） of Collagense VJ/heparini solution （2μl）. EA （2 Hz, 1 mA） was applied to ＂Baihui＂ （GV 20）-＂Taiyang＂ （EX-HN 5） for 30min. Chondriosome suspension of the cerebral perihemotoma tissue was got by gradient centrifugation for detecting SDH and Na^＋-K^＋-ATPase activity, and LA content with chro- mometry. Results In comparison with sham-operation group and control group, SDH and mitochondrial Na^＋-K^＋--ATPase activi- ty in cerebral peri-hemotoma tissue declined significantly from 72 h and 24 h on respectively （ P〈0. 05）, while LA level increased markedly from 24 h on （P〈0.05） in model group. Compared with sham-operation group, SDH activity （ 168 h） and Na^＋-K^＋-- ATPase activity （24 h, 72 h and 168 h） in EA group also decreased significantly （P〈0.05） ; LA level （24 h, 72 h） in EA group increased considerably （P〈0.05）. Whereas compared with model group, SDH and Na^＋-K^＋-ATPase activity increased significantly at most time courses （ P〈0.05）, and I_A level decreased remarkably （P〈0.05） in EA group. Conclusion EA can improve the energy metabolism, upregulate the activity of chondriosome,and reduce the accumulation of LA in the cerebral peri-hemotoma tissue of ICH rats.