电针对血管性痴呆大鼠血浆和脑组织生长抑素、β-内啡肽含量及学习记忆能力的影响

Effects of Electroacupuncture on Plasma and Cerebral Somatostatin and β-EP Contents and Learning-memory Ability in Vascular Dementia Rats

目的:观察电针对血管性痴呆(VD)大鼠学习记忆能力的影响,探讨电针的治疗作用机制。方法:雄性SD大鼠,随机分为假手术组(n=8)、模型A组(n=8)、模型B组(n=8)、电针组(n=9)、西药组(n=8)。采用4-血管阻断法制作脑缺血模型。电针"百会""大椎""脾俞""肾俞"穴,施以连续波,频率150 Hz,强度约1 mA,留针20 min,每日1次,连续治疗15 d。尼莫通治疗按12 mg/kg、20 ml/kg灌胃。以Morris水迷宫测定大鼠学习记忆能力,并用放射免疫法测定血浆和脑组织生长抑素(SS)、β-内啡肽(-βEP)的含量。结果:模型组大鼠血浆和脑组织中SS的含量及脑组织中-βEP的含量显著降低,与假手术组相比差异有显著性意义(P<0.01),血浆中-βEP含量无明显变化(P>0.05)。模型组大鼠表现明显的学习记忆能力障碍,在水迷宫试验中,其潜伏期显著延长(P<0.01),在原平台象限跨越平台次数与其余3个象限差异无显著性意义(P>0.05)。经电针和西药治疗后潜伏期明显缩短(P<0.01),相同时间内跨越原平台次数明显多于其余3个象限(P<0.01);其血浆和脑组织中SS含量及脑组织中-βEP含量显著升高(P<0.01)。结论:电针能改善VD大鼠血浆和脑组织中的SS、-βEP含量,从而提高VD大鼠学习记忆能力。

Objective To observe the effect of electroacupuncture （EA） on learning-memory ability of vascular dementia （VD） rats, and the simultaneous changes of plasma and cerebral somatostatin （SS） and lS-endorphin （EP） contents. Methods Forty-one SD rats were randomly divided into control （n = 8）, model A （n = 8, no treatment） and B （n = 8, intragastric perfusion of 15 % saline）, EA （n = 9） and medication （n = 8, intragastric perfusion of Nimoldipine, 12 mg/kg） groups. VD model was established by using modified 4-vessels occlusion method. EA （150 Hz, 1 -2 mA） was applied to ＂Baihui＂ （GV 20）, ＂Dazhui＂ （DU 14）, ＂Pishu＂ （BL 20） and ＂Shenshu＂ （BL 23） for 20 min, once daily for 15 days. Morris water maze tests were conducted for evaluating the rats＇ learning-memory ability, The contents of SS and β-EP in plasma and brain tissue were measured by radioimmunoassay （RIA）, Results In comparison with sham-operation group, the escape latency （EL） prolonged significantly and the target-platform crossing times decreased remarkably （P〈0.01 ） in model group B. In comparison with model group β-EL shortened and target-platform crossing times increased both significantly in EA and medication groups （P〈0.01 ）, Plasma and cerebral SS, and cerebral β-EP contents of model groups A and B were significantly lower than those of shamperation group（ P〈0. 01 ）, while plasma β-EP level had no obvious change （P〉0. 05）, Plasma and cerebral SS, and cerebral β-EP contents in both EA and medication groups were considerably higher than those in model groups A and B （P〈0.01 ）, No significant differences were found between EA and medication groups in EL, target-platform crossing times, plasma and cerebral SS and β-EP levels, and between model group A and model group B in plasma and cerebral SS and β-EP levels （P〉0.05）. Conclusion EA can raise plasma and cerebral SS and cerebral β-EP levels, and improve the learning-memory ability in VD rats.