CARP相互作用蛋白HTRM的表达谱分析及亚细胞定位

Expression Analysis and Subcellular Localization of HTRM,a Association Protein of CARP

目的观察CARP相互作用蛋白HTRM的组织表达谱和亚细胞定位及其对血管平滑肌细胞(VSMC)增殖的作用。方法我们利用CLONTECH公司的成人多组织膜,通过Northern杂交的方法,检测HTRM在心脏、脑、胎盘、肺、肝、骨骼肌、肾脏以及胰腺中的表达情况。将HTRM带上GFP标签转染HEK293S细胞,观察其亚细胞定位情况。再将HTRM及对照载体转染VSMC细胞,通过细胞计数以及MTT两种方法检测细胞的增殖情况。结果Northern Blot实验显示HTRM有两个转录本,2.4kb的转录本主要在心脏和骨骼肌中表达,1.35kb的转录本主要在胎盘中表达;亚细胞定位显示HTRM是一个胞浆蛋白;高表达HTRM可以轻微的促进VSMC细胞增殖。

Objective To test the tissue distribution and subcellular localization of HTRM. Methods Northern blot was used to detect the tissue distribution of HTRM in heart, brain, placenta, lung, liver, skel- etal muscle, kidney and pancreas. HEK293S cells were transfected with pGFP-HTRM or control vector （pG- FP）, and fluorescence was performed with a confocal microscope. The influence of over-expression of HTRM on VSMC cell growth was also assayed by cell counting and MTT assay. Results HTRM was predominantly expressed in normal heart （2.4 kb）, skeletal muscle （2.4 kb）, and placenta （1.35 kb）. HTRMM was a cytoplasmic protein, and Over-expression of HTRM in VSMC could promote the cell proliferation shghtly.