溃疡性结肠炎中医辨证分型与黏附分子关系的研究

Correlation between TCM differentiation of syndromes typing for ulcerative colitis and coherent molecules

目的研究溃疡性结肠炎(UC)患者部分黏附分子表达和中医辨证分型的关系。方法将溃疡性结肠炎患者(UC组)35例辨证分为大肠湿热证(16例)、肝郁脾虚证(8例)、脾肾阳虚证(6例)、血瘀肠络证(5例),用流式细胞术测定各个证型患者外周血中吞噬细胞膜糖蛋白CD44、可溶性黏附分子P-选择素(CD62P)及可溶性细胞间黏附分子-1CD54的表达情况,并与正常对照组进行比较。结果大肠湿热证及血瘀肠络证的CD44、CD62P、CD54表达最高,肝郁脾虚证、脾肾阳虚证次之,与正常对照组比较差异均有统计学意义(P<0.01),大肠湿热证及血瘀肠络证间比较差异无统计学意义(P>0.05),与其他2个证型比较差异均有统计学意义(P<0.01)。肝郁脾虚证及脾肾阳虚证间比较差异无统计学意义(P>0.05)。结论CD44、CD62P、CD54表达与中医辨证分型有关,测定外周血中CD44、CD62P、CD54表达情况可以协助中医辨证分型,确定治疗方案。

Objective To study the correlation between TCM differentiation of syndromes typing for ulcerative colitis （UC） and expression of coherent molecules. Methods 35 patients with UC were divided into 5 groups according to TCM differentiation of syndromes including Dachangshire syndrome group（ 16 cases）, Ganyupixu syndrome group （8 cases）, Pishenyangxu syndrome group （6 cases）, Xueyuchangluo syndrome group （5 cases）. The expression of CD44, CD62P, CD54 in patients＇ peripheral blood in different syndromes were detected and compared. Results The expressions of CD44, CD62P, CD54 were highest in Dachangshire syndrome group and Xueyuchangluo syndrome group, next Ganyupixu syndrome group and Pishenyangxu syndrome group, there were significant differences between the 4 groups and normal control group（ P 〈 0.01 ）, however, there was no significant difference between Dachangshire syndrome group and Xueyuchangluo syndrome group（ P 〉 0.05）, there were significant differences between Dachangshire syndrome group as well as Xueyuchangluo syndrome group and Ganyupixu syndrome group and Pishenyangxu syndrome group（ P 〈 0.01 ）. But there was no significant difference between Ganyupixu syndrome group and Pisilenyangxu syndrome group（ P 〉 0.05）. Conclusion The expres- sion of CD44, CD62P, CD54 is closely related to TCM typing according to differentiation of syndromes, and the detection of expression of CD44, CD62P, CD54 in patients＇ peripheral blood is helpful to the TCM typing according to differentiation of syndromes and to selection of treatment plan.