胶质母细胞瘤细胞系GL15生物学特性的实验研究

The experimental study of relevant biological characterization of glioblastoma cell line GL15

目的明确GL15细胞系的生物学特征并探讨表皮生长因子(EGF)对GL15细胞系生物学特征的影响。方法观察GL15细胞的形态和生长增殖特性,Giemsa染色分析核型,免疫细胞化学行胶质纤维酸性蛋白(GFAP)、增殖细胞核抗原(PCNA)染色,流式细胞仪分析细胞周期,Transwell、MTT法检测EGF对GL15细胞生物学特征的影响,与U251MG和U373MG细胞系进行比较。结果GL15细胞为典型的胶质母细胞瘤形态,核型分析显示多条染色体均有扩增,其中EGF受体所在的7号染色体数目增加较为明显(4条7号染色体)。GFAP和PCNA分别在胞浆和胞核呈阳性表达。EGF可双向调节细胞增殖活性、侵袭性及GFAP蛋白表达。结论GL15细胞系保持了胶质母细胞瘤的生物学特征,是研究胶质母细胞瘤理想的细胞平台。该细胞系的生物学特性与EGF受体有紧密关系。

Objective To explicate the biological character of glioblastoma cell line GL15 and to study the effect of epidermal growth factor （EGF） to the biological character of GL15 cell line. Methods The morphology, growth and proliferation of the glioblastoma cell line GL15 was observed during continuous culture in vitro. The karyotype of GL15 cells with Gimsa stain was analyzed. The expression of glial fibrillary acidic protein（GFAP） and proliferating cell nuclear antigen（PCNA） protein were detected with immuocytochemistry, the cell cycle was detected by flow cytometry（FCM）, the effect of EGF on the biological character of GL15 cell line was measured by methyl thiazolyl tetrazolium （MTT） and Transwell, and campared with these characterizations of U251MG and U373MG glioblastoma cell line. Results The morphology of GL15 cells are typical glioblastoma cell morphology. NO. 7 chromosome, EGFR located, is over amplification with Gimsa stain. They grew and proliferated well in cell culture eondition and were able to invade and form cell clone in vitro. The expression of GFAP and PCNA are positive, mainly in kytoplasm and nuclei respectively. EGF can regulate the cell proliferation, invasive ability and GFAP expression of GL15. Conclusion GL15 cell line remains the biological character of typical glioblastoma cell character, it is an ideal cell platform to study glioblastoma character. Its biological characterization are intimate correlated to EGFR.