铜绿对大鼠的急性毒性作用

Study on Acute Toxicity of Patina on Rats

[目的]探讨铜绿对大鼠的急性毒性,计算其半数致死剂量(LD_(50))。[方法]选用90只健康成年SD大鼠,雌雄各半,染毒剂量依次为0、217.3、325.9、488.9、625.0、733.3、1 100.0、1 250.0和1 775.0mg/kg。经口24h内3次灌胃染毒,观察14 d,记录大鼠的毒性反应和死亡数,以改良寇式法计算LD_(50),并进行肝、胃、肾病理学检查。[结果]染毒后不同时间,大鼠自由活动减少,摄食和饮水下降,出现腹泻等症状。铜绿的LD_(50)及95%可信区间(95%CI)为732.82 (870.96～615.18)mg/kg。病理学检查发现存活动物均出现肝细胞浊肿、坏死等。在本次实验剂量下,铜绿未引起胃和肾脏的病理改变。[结论]铜绿的毒性属于低毒,肝脏为铜绿的急性毒性靶器官。

[Objective ] To investigate the acute toxicity of patina in rat and calculate its 50% lethal dose（ LD50 ）. [ Methods ] 9 groups of mature SD rat were employed for testing the acute toxicity of patina, at the doses of 0, 217.3, 325.9,488.9,625.0, 733.3, 1 100.0, 1250.0 and 1 775.0mg/kg respectively. The rat were administered with patina three times in 24 hours and observed for 14 days. The behavior and the toxic symptoms were observed, and the lethal dose 50 s（ LD50 ）was calculated by the modified Karber＇ s method, morphological changes of liver, stomach and kidney were also examined. [ Results ] The frequency of active behavior of rats reduced after patina was given. Before death, rats＇ eating and drinking were decreased, accompanied with diarrhea. The LD50（ 95%CI）of patina was 732.82（ 870.96-615.18 ）mg/kg. Patina caused obvious swell and necrosis in liver cell of rats. No obvious morphological changes of stomach and kidney after administration of patina were observed. [ Conclusion ] The results indicated that patina is a matter with low toxicity. There was no statistical difference in mortality between female and male rats. It suggests that liver is the acute toxic target organ of patina.