2，4-二硝基氯代苯对小鼠骨髓源树突状细胞膜表面分子的影响

Membrane Surface Molecules Expression on Mice Dendritic Cells Derived from Bone Marrow Induced by 2,4-dinitrochlorobenzene

[目的]筛选小鼠骨髓来源树突状细胞(DC)在化学物致敏性评价中的合适指标。[方法]将皮肤致敏物2,4-二硝基氯代苯(DNCB)和皮肤刺激物十二烷基磺酸纳(SDS)分别作用于小鼠骨髓来源DC,设立对照组,在不同的时段,用流式细胞仪检测DC膜表面分子CD80、CD86和MHCⅡ类分子。[结果] CD80在SDS染毒后,48h和60h时表达增幅最大,约335%,而DNCB组和对照组则平稳增加,最大增幅分别为50%和60%;CD86表达在DNCB染毒48h时达到最大值,增加约262%,而SDS组和对照组最大增幅分别为72%和66%,12h后趋于降低;MHCⅡ类分子表达则在3组中均出现大幅波动。[结论] CD86分子表达在化学物致敏性评价中有一定的应用前景。

[ Objective ] To screen applicable indicators in assessing potential of sensitizing chemicals using mice dendritic cells （ DC ） derived from bone marrow. [ Methods ] Mice DC derived from bone marrow were administrated with the chemical sensitizer 2, 4-dinitrochlorobenzene （ DNCB ） and the skin irritant sodium dodecyl sulphate （ SDS ）, respectively. Membrane molecules CD80, CD86 and MHC class Ⅱ were evaluated by flow cytometer after treatment for different times. [ Results ] CD80 were most significantly upregnlated at 48 h and 60 h after SDS-treated （ increased by 335% ）, while increased smoothly in the DNCB-treated and control group（ increased by 50% and 60%, respectively ）. CD86 were most significantly upregnlated after DNCB treatment for 48 h（ increased by 262% ）, while the maximums in SDS-treated and control group were after administration for 12 h（ increased by 72% and 66%, respectively ）. MHC class Ⅱ fluctuated significantly among all the three groups. [ Conclusion ] CD86 might be used as a marker in the assessment of sensitization induced by chemicals.