中国对虾细菌性黑鳃病的组织病理观察

HISTOPATHOLOGICAL OBSERVATION OF BLACK GILL DISEASE IN PENAEUS CHINENSIS

报道中国对虾细菌性黑鳃病的症状、鳃组织的显微、超显微结构的病理变化。病虾鳃区呈黑色条斑状、活动力差、厌食、死亡率较高。光镜下观察，鳃组织严重坏死、断裂，鳃丝充血，为多量血淋巴细胞浸润，黑色素大多沉积在鳃丝末端。电镜观察到鳃组织的甲壳层和表皮层中有许多不同切面的杆状细菌，呈多形态，大小约为（0．2～0．4）×（1.2～1．8）微米，单个，偶而连成双，无芽孢，菌体周围细胞结构完全瓦解，组织被腐蚀成许多圆形或不规则的空斑。

This paper reports symptoms and histopathological of black gili disease in penaeus chinensis infected with bacteria. The diseased shrimp appeared black streaky gills, anorexia,lethargy and a high mortality. From light microscopic observation,gill filaments showed serious necrosis, brocking, hyperaemia and infiltration of many hemolymphatic cells. Melanins were mostly observed in the tip of gill filaments. Electron microscopic examination revealed that numerous bacteria of different sections were presented in cuticular and epithelial layers of the gills. The pathogen was rod shaped, no-gemma, pleomorphic forms, 0. 2 ～0. 4μ wide and 1. 2 ～1. 8 μ long, single or occasionally united into pairs. The structures of eells were completely disintegrated around the bacteria,and infected tissue was heavily necrosed and formed a lot of round or irregular vacuous spots.