成年房间隔缺损患者核因子κΒ活化与细胞因子分泌的相关性研究

Relationship between nuclear factor κΒ activation on peripheral blood mononuclear cell and serum cytokines levels of adult patients with atrial septal defect

目的探讨成年房间隔缺损(atrial septal defect,ASD)患者外周血单个核细胞(PBMCs)核因子κΒ(NF-κΒ)表达与肿瘤坏死因子α(TNF-α)、白细胞介素6(IL-6)分泌的相关性。方法30例ASD患者和30例健康对照组PBMCs NF-κΒ的表达由免疫组化染色检测,血清细胞因子的含量由酶联免疫吸附试验(ELISA)测定。对两组的指标进行t检验及相关分析。结果ASD患者PBMCs NF-κΒ胞核染色阳性率和血清TNF-α、IL-6含量显著高于健康对照组,分别为(43.67±19.16)%vs(22.97±12.77)%,(0.79±0.11)μg/L vs(0.23±0.09)μg/L,(0.46±0.07)μg/L vs(0.03±0.02)μg/L,均P<0.01,且NF-κΒ胞核染色阳性率和血清TNF-α、IL-6含量呈显著正相关(rs=0.783,0.589,均P<0.01)。结论成年ASD患者NF-κΒ表达增高,细胞因子TNF-α及IL-6分泌增多。在成年ASD,炎症基因转录激活,可能通过NF-κΒ表达的上调致使炎性细胞因子(TNF-α、IL-6)的增加。

Objective To study the relationship of nuclear factor-kappa B（NF-κB） activation on peripheral blood mononuclear cells（PBMCs） and the levels of tumor necrosis factor（TNF-α）,interleukin 6 （IL-6） of adult patients with atrial septal defect. Methods Thirty atrial septal defects in adults and 30 control subjects,matched in age and gender, were included. TNF-α and IL-6 concentrations were measured with quantitative enzyme-linked immunosorbent assay （ELISA）. The activity of NF-κB was measured by immunocytochemical staining. Results Compared with those of healthy subjects, NF-κB activation, TNF-α and IL-6 levels of adult patients with atrial septal defect increased significantly （43.67±19.16）% vs （22.97± 12.77） %, （0.79±0.11）μg/L vs （0.23±0.09） μg/L, （0.46±0.07） μg/ L vs （0.03±0.02） μg/L,respectively （all P 〈0.01）. NF-κB activation was positively correlated with the levels of TNF-α and IL-6 （ r, =0. 783,0. 589,all P 〈0.01）. Conclusion NF-κB activation,the levels of TNF-α and IL-6 are increased in adult patients with atrial septal defect. NF-κB possibly up-regulates the levels of TNF-α and IL-6 of adult patients with atrial septal defect.