利用双目标组织建立高频大豆再生体系的研究

Establishment of High Frequency Regeneration of Soybean by Binary Target Tissues

利用双目标组织(即一个外植体同时含有子叶节和初生叶节两个目标组织)进行了大豆高频再生体系建立的研究,以便为高效大豆转基因技术体系的建立提供技术保障。结果表明,不同基因型之间大豆不定芽诱导率不同,在所试的5个大豆品种中,初生叶节不定芽诱导率最高的品种是合丰35,合丰25次之,分别达到50.0%,46.9%;而子叶节不定芽诱导率最高的品种是合丰25,为75.0%;总的不定芽诱导率最高的品种是合丰25,达到121.9%。不同浓度6-BA对丛生芽的诱导率在不同品种间有差异,6-BA浓度为1.5 mg/L时,合丰35的丛生芽诱导率最高,平均每个外植体诱导出6.7个丛生芽。

Soybean regeneration system was studied using an explant containing cotyledonary node and primary leaf node as the target tissue in the paper. The results showed that regeneration frequency of adventitious shoots was different among 5 soybean varieties tested. The variety with the highest regeneration frequency from the primary leaf node was Hefeng 35,reached up to 50.0%, and the variety with the highest regeneration frequency from the cotyledonary node was Hefeng 25;whereas the variety with the highest regeneration frequency from both the cotyledonary node and the primary leaf node was Hefeng 25, reached up to 121.9 ~. The inducting rate of different 6 BA concentration on multi-shoots was different among different variety. The inducting rate of the multi-shoots from Hefeng 35 was the highest when the concentration of 6-BA was 1.5 mg/L.