组蛋白去乙酰化酶3在急性白血病细胞株中的表达

Expression of histone deacetylase 3 in acute leukemic cell lines

目的:研究组蛋白去乙酰化酶3(histone deacetylase 3,HDAC3)在不同类型急性白血病细胞株中的表达,观察HDAC3表达和细胞分化增殖的关系。方法:在16株白血病细胞株中采用实时定量PCR法及Western印迹法检测HDAC3的mRNA及蛋白质表达,检测NB4细胞株在维甲酸诱导分化过程中HDAC3 mRNA及蛋白质表达变化情况。结果:在NB4、NB4RA和NOMO-1细胞株中HDAC3 mRNA表达量较高,但蛋白质的表达与此不一致。在维甲酸诱导的NB4细胞分化过程中HDAC3 mRNA和蛋白质的表达呈下降趋势。结论:HDAC3的表达是白血病细胞生长所必须,在NB4细胞中HDAC3表达和细胞的分化和增殖状态相关。

Objective ：To study the expression of histone deacetylase 3 （HDAC3） in acute leukemic cell lines and to observe whether the HDAC3 expression is necessary for the transcriptional regulation by controlling proliferation and differentiation of leukemia cells. Methods：The HDAC3 mRNA and protein expression levels in 16 acute myeloid leukemic cell lines were assessed by real-time quantitative PCR （RTQ-PCR） and Western blotting, respectively. The mRNA and protein expression levels of HDAC3 were detected during differentiation of NB4 cells induced by all-trans retinoic acid （ATRA）. Results：The mRNA expressions of HDAC3 were higher in NB4, NB4RA and NOMO-1 leukemic cell lines but the protein expression levels were not parallel with mRNA expressions. HDAC3 mRNA and protein expression had a decreased tendency during the ATRA-induced differentiation of NB4 cells. Conclusion ： HDAC3 is necessary for proliferation of leukemic cells and the expression of HDAC3 in NB4 cell line is associated with cell differentiation and/or proliferation.