口腔鳞癌诱导型一氧化氮合酶mRNA的表达及其临床意义

Expression of inducible nitric oxide synthase mRNA in oral squamous cell carcinoma and its clinical significance

目的:探讨诱导型一氧化氮合酶(inducible nitric oxide synthase,iNOS)在口腔鳞癌(oral squamous cell carcinoma,OSCC)发生、发展中的作用及其与OSCC的临床病理特征之间的关系。方法:运用原位杂交技术检测10例口腔正常黏膜、12例单纯增生上皮、28例不典型增生上皮及32例鳞癌组织中iNOS mRNA的表达情况。结果:正常口腔黏膜组织缺乏iNOSmRNA表达;不典型增生组和鳞癌组中iNOS mRNA的表达较单纯增生组均显著增加(P<0.001);随着上皮不典型增生程度的加重,iNOS mRNA表达显著增强(P<0.05);不典型增生组与鳞癌组之间iNOS mRNA的表达差异无统计学意义(P>0.05)。高、中、低分化的3种鳞癌组织中iNOS mRNA表达差异有统计学意义(P<0.05)。口腔鳞癌患者Ⅲ、Ⅳ期组与有颈淋巴结转移组iNOS mRNA表达分别明显高于Ⅰ、Ⅱ期组与无颈淋巴结转移组(P<0.05)。结论:iNOS mRNA可能在口腔鳞癌的发生、发展中起重要作用;口腔鳞癌的组织分化程度、TNM分期及颈淋巴结转移与iNOS mRNA表达关系密切。iNOS的检测可能对口腔鳞癌患者的预后判定和指导治疗具有参考意义。

Objective：To study the role of mRNA expression of inducible nitric oxide synthase （iNOS） in the occurrence and development of oral squamous cell carcinoma （ OSCC ） and the relationship between the expressions of iNOS mRNA and the clinical pathologic characteristics of OSCC. Methods： In situ hybridization assay was used to detect iNOS mRNA in 10 cases of normal oral mucosa, 12 cases of oral epithelial simple hyperplasia, 28 cases of oral epithelial atypicall hyperplasia, and 32 cases of OSCC. Results： The expressions of iNOS mRNA was negative in the normal oral mucosa. The expression of iNOS mRNA in both atypical hyperplasia and OSCC groups were significantly higher than that in simple hyperplasia group （P 〈 0.001 ）. Statistical analysis showed a significant correlation between iNOS mRNA expression and the grade of atypical hyperplasia （ P 〈 0.05 ）. No significant difference of iNOS mRNA expression was found between atypical hyperplasia and OSCC groups （ P 〉 0.05 ）. The expression of iNOS mRNA in the group of well-, moderately- and poorly-differentiated squamous cell carcinoma was significantly different （ P 〈 0.05 ）. The expression of iNOS mRNA in stage Ⅲ and Ⅳ OSCC tissues was significantly higher than those at stage Ⅰand Ⅱ, and significantly higher in OSCC patients with neck lymph node metastasis than those without neck lymph node metastasis （P 〈 0.05 ）. Conclusion： iNOS mRNA may play an important role in OSCC development and progression. The expression of iNOS has a close relationship with the differentiation, TNM stage, and neck lymph node metastasis of OSCC. Detection of NOS may be useful in evaluation of patient＇ s prognosis and guidance of OSCC treatment.