瘦素及其功能性受体OB-Rb在非小细胞肺癌表达的临床病理研究

Clinicopathologic research on the expression of leptin and its fuctional receptor OB-Rb in non-small cell lung cancer

目的:研究瘦素和瘦素功能性受体OB-Rb在非小细胞肺癌(non-small cell lung cancer,NSCLC)中的表达,并探讨瘦素和OB-Rb在NSCLC发生、发展过程中的意义。方法:采用RT-PCR和免疫组织化学方法,检测38例NSCLC肿瘤组织及其癌旁组织中瘦素和瘦素受体OB-Rb的mRNA和蛋白表达情况,同时将其蛋白表达水平与NSCLC的临床病理参数进行相关性分析。结果:瘦素与OB-Rb的mRNA在肺癌组织表达水平显著高于癌旁组织(P=0.000)。瘦素蛋白在肺癌组织和癌旁组织的阳性表达率分别为71.1%和26.3%(P<0.005),OB-Rb蛋白在肺癌组织和癌旁组织的阳性表达率分别为63.2%和31.6%(P<0.010);瘦素和OB-Rb蛋白与NSCLC细胞类型、分化程度、TNM分期及淋巴结转移无明显相关性(P>0.05),瘦素与OB-Rb蛋白在NSCLC中的表达也无显著关联(P>0.05)。结论:瘦素和OB-Rb在肺癌组织表达上调,瘦素可能是NSCLC发生、发展过程中的一种促肿瘤生长因子。瘦素和OB-Rb在NSCLC的促癌作用没有直接的协同效应,可能是通过各自不同的机制参与NSCLC的病程。

Objective： To determine the expression of leptin and its fuctional receptor OB-Rb in non-small cell lung cancer （NSCLC） and investigate their role in carcinogenesis and development of NSCLC. Methods： Reverse transcription polymerase chain reaction （RT-PCR） and immunohistochemical staining were used to evaluate mRNA and protein expressions of leptin and OB-Rb in 38 NSCLC tumor tissues and the corresponding adjacent lung tissues. The relationship between the protein expressions of leptin and OB-Rb and clinicopathological parameters was analyzed. Results：The mRNA expression level of leptin and OB-Rb in lung cancer tissues was significantly higher than that in adjacent normal lung tissues （P=-0.000 ）. The positive rate of leptin protein was 71.1% and 26.3 % in NSCLC tissues and adjacent noncancerous tissues, respectively （ P 〈 0. 005 ）. The positive rate of OB-Rb protein was 63.2% and 31.6% （P 〈 0.010）. The expressions of leptin and OB-Rb had no correlation with histological subtypes, differentiation, TNM staging, or lymph node metastasis of NSCLC （ P 〉 0.05 ）. There was no relationship between leptin and OB-Rb protein over-expression in NSCLC （P 〉 0.05 ）. Conclusion： The expressions of leptin and OB-Rb in NSCLC were up-regulated suggesting that leptin may be identified as a potential stimulating factor in carcinogenesis and development of NSCLC. There was no directly synergestic effect between leptin and OB-Rb in the process of NSCLC. Leptin and OB-Rb may be independently involved in the pathogenesis of NSCLC by different mechanisms.