皂荚提取物对人肝癌细胞相关癌基因表达的调控作用及端粒酶的影响

Influence of Glelitsia sinensis Lam extractive on the regulation of cancerous gene and telomerase activity of human hepatocarcinoma cell

目的:研究皂荚提取物对人肝癌细胞的增殖、相关癌基因表达的调控作用及端粒酶的影响。方法:体外培养人肝癌细胞bel-7402,检测不同浓度皂荚提取物(0.05mg/ml、0.1mg/ml、0.2mg/ml)对人肝癌细胞bel-7402细胞增殖、细胞周期、肝癌相关基因(bax、bcl-2、p53)的表达及端粒酶活性的影响。结果:0.05mg/ml～0.2mg/ml浓度组皂荚提取液对人肝癌细胞bel-7402细胞增殖、调控癌基因与抑癌基因、促进癌细胞凋亡、抑制端粒酶活性差异均具有显著性意义(P<0.05或P<0.01)。结论:皂荚提取物能够抑制人肝癌细胞增殖,促进细胞凋亡。

Objective： To study the influence Glelitsia sinensis Lam extractive on the proliferation, the regulation of cancerous gene and telomerase activity of human hepatocarcinoma cell. Methods： Bel-7402 cell was cultured in vitro and treater with different concentration of Glelitsia sinensis Lam （0. 05mg/ml, 0. lmg/ml, 0. 2mg/ml） . MTT was explored to detect the praliferation of bel-7402 cells. Flow cytometry was used to measure apptosis and cellcycle. RT-PCR and immunohistochemical method was adapted to assay the expressionef cancerousgene. Results： The coneentration of Glelitsia sinensis Lam from 0. 05mg/ ml to 0. 2mg/ml could manedkly inhibit the praliferation, the expression of cancerous gene and the thlomerase activity （ P 〈 0. 01 ） . Conclusion ： The extractive of Glelitsia sinensis Lam can control the proliferation, accelerate the apoptotsis of bel-7402 cell.