鼻咽癌口腔黏膜急性放射损伤与p53基因多态的关系

Relation Between Radiotherapy-induced Acute Injury of Mucosa of Nasopharyngeal Carcinoma and p53 Polymorphisms

目的探讨p53基因的单核苷酸多态与鼻咽癌(NPC)口腔黏膜急性放射损伤的关系。方法以PCR-RFLP (聚合酶链反应-限制性片断长度多态性)分析方法,对56例单纯放疗或同步放化疗的NPC患者,进行p53的72密码子Arg-Pro多态检测,并对口腔黏膜的急性放射损伤评级。结果携带72Pro等位基因的患者的口腔黏膜急性放射损伤程度与未携带72 Pro等位基因的患者之间的差异无统计学意义(P>0.05);单次剂量大与同步放化疗患者的口腔黏膜急性放射损伤的程度明显增加,差异有统计学意义(分别P<0.01和P<0.05)。结论鼻咽癌患者p53的72密码子Arg-Pro基因型与口腔黏膜急性放射损伤无关。

Objective To explore the relation between p53 genetic polymorphisms and radiothera- py-induced acute injury of mucosa of oral cavity mucosa. Methods The total of 56 patients with NPC treated by radiotherapy alone or with chemoradiotherapy synchronically were genotyped for the p53 codon 72 Pro-Arg SNP using PCR-RFLP assays, and were ranked according to the acute injury of oral cavity mucosa. Results There was no difference in acute injury of oral cavity mucosa between the p53 Pro allele carriers and the other carriers（P〉0.05）; the high single dose（P〈0.01 ） and concomitant chemoradiotherapy（P〈0.05） resulted in increase in acute injury of oral cavity mucosa. Conclusion Those results suggest that p53 SNP may not associate with radiotherapeutic acute injury of oral cavity mucosa.