p16蛋白在宫颈尖锐湿疣和鳞癌中的表达及其临床意义

The Expression and Clinical Significance of p16 in Cervical Condyloma Accuminatum and Cervical Squamous Cell Carcinoma

目的探讨p16蛋白在宫颈尖锐湿疣(CA)和鳞癌组织中的表达及其临床意义。方法应用免疫组织化学s-p法检测40例宫颈CA、40例宫颈鳞癌和10例正常宫颈组织中p16的表达情况。结果p16蛋白在宫颈鳞癌、宫颈CA、正常宫颈组织中的阳性表达率分别为90%、35%、30%,宫颈鳞癌显著高于正常宫颈、宫颈CA组织,差异有高度统计学意义(均P<0.01);宫颈CA与正常宫颈组织相比,差异无统计学意义(P>0.05)。p16蛋白的表达与宫颈鳞癌有无淋巴结转移呈正相关(P<0.05),与临床分期、组织学分级无相关性(P<0.05)。结论p16蛋白表达的增高可能出现在宫颈病变的早期,与细胞恶性转化有关,对p16表达强阳性的宫颈CA对应积极治疗并加强随访。

Objective To investigate the expression and clinical significance of p16 in cervical condyloma accuminatum and cervical squamous cell carcinoma. Methods Imunohistochemical staining was performed with s-p method to detect the expression of p16 proteins in the epithelial tissue of 40 cases of cervical condyloma accuminatum and 40 cases of cervical squamous cell carcinoma, 10 cases of normal cervix as the controls. Results The positive staining of p16 in cervical squamous cell carcinoma, cervical condyloma accuminatum and normal cervix was 90%, 35% and 30% respectively. The expression of p16 in cervical squamous cell carcinoma was significantly stronger than that in cervical condyloma accuminatum and normal cervical tissue（P〈0.01）. There was no significant difference existing between cervical condyloma accuminatum and normal cervix （P〉0.05）. The expression of p16 was significantly related to lymphatic metastasis（P〈0.05）; however, it had no statistical relation to the clinical phases and histopathological stages（P〉0.05）. Conclusion It is possible to predict the canceration of cervical condyloma accuminatum and diagnose it in early stage, by detecting the expression of p16 in tissue of condyloma accuminatum clinically.