HPLC测定宁心宝胶囊中核苷类化合物含量

Determination of nucleosines in Ningxinbao Capsules by HPLC

目的：建立高效液相色谱法测定宁心宝胶囊（真菌虫草头孢）中核苷类化合物的含量。方法：采用十八烷基键合硅胶色谱柱（4．6mm×250mm，5 μm）；以磷酸盐缓冲液（pH7．8～8．0）-乙腈（83：17）为流动相，流速为1．0mL／min；检测波长为260nm。结果：宁心宝胶囊中主要成分尿苷在4．168～83．36 μg／mL范围内有良好的线性关系（r=0．9999）；腺苷在4．480-89．60 μg／mL范围内有良好的线性关系（r=0．9999）；次黄嘌呤核苷在5．230-104．6 μg／mL范围内有良好的线性关系（r=0．9999），平均加样回收率分别为99．6％，102．1％和111．7％，RSD分别为0．4％，1．8％和2．5％。结论：此法简便、快速。适用于宁心宝胶囊中核苷类化合物含量的同时测定，为控制其质量提供了简便易行的方法。

AIM： To establish HPLC method for making a assay of nucleosines in Ningxinbao Capsules （Cepha- lospovium sinensis Chen. sp. nov）. METHODS： The assay was performed on a C18 column （4.6 mm ×250 mm,5 μm）. The mobile phase was composed of phosphate buffer （pH 7.8 -8.0）-acetonitrile （83： 17）. The flow rate was at 1.0 mL/min. The detection wavelength was at 260 nm. RESULTS： The linear ranges of uridine, adenosine and inosine lay in the concentration range of 4. 168 -83.36, 4. 480 -89.60, 5. 230 - 104.6 μg/mL, respectively （ r = 0. 999 9）. The average recoveries were 0.4%, 1.8% ,2.5 %, respectively. CONCLUSION ： The method is simple, rapid. It is suitable for the assay of nucleosines in Ningxinbao Capsules.