摘要
凡纳滨对虾为实验对象,进行了抗生素和有益菌对对虾免疫效应的研究.抗生素组每天泼洒氯霉素1次,浓度约为2mg/dm3;有益菌组每隔7d泼洒1次有益微生物,包括利生活菌90g和EM18cm3.实验为期1个月,每10d取样1次,检测凡纳滨对虾血清、肌肉和肝脏中的溶菌力、抗菌力、酚氧化酶、碱性磷酸酶、超氧化物歧化酶.结果表明,除个别情况外,抗生素组的凡纳滨对虾血清、肌肉和肝脏中的溶菌力、抗菌力、酚氧化酶、碱性磷酸酶、超氧化物歧化酶低于对照组,而有益菌组则高于对照组.由此可以推断,抗生素对凡纳滨对虾非特异性免疫系统具有一定的抑制作用,而有益菌则可以从一定程度上提高凡纳滨对虾非特异性免疫水平.
Effects of antibiotic and probiotic on nonspecific immune system of Pacific white shrimp L/topenaeus vannamei were investigated. Antibiotic group was treated with chloramphenicol at 2mg/dm^3, and probiotic group was treated with 90g probiotic and 18cm^3 every 7 days. Each treatment was run in duplicate and the experiment lasted for a month. Sampling was made every 10 days. The bacteriolytic activities （ UL）, antibacterial activities （U.）, phenoloxidase activities （PO）, alkaline phosphatase activities （ALP） and superoxide dismutase activities in the serum, muscle and hepatapancreas were determined to investigate the effects of humoral immune factors on L. vannamei. The results showed that chloramphenicol could inhibit the nonspecific immune system of L. vannamei, but probiotics could improve the nonspecific immune system of L. vannamei.
出处
《台湾海峡》
CAS
CSCD
北大核心
2008年第2期161-167,共7页
Journal of Oceanography In Taiwan Strait
基金
福建省重大项目(2002I003)
福建省青年科技人才创新基金资助项目(2004J055)
福建省自然科学基金资助项目(Z0515011)
国家海洋局第三海洋研究所所长基金资助项目(海三科05003、海三科05007)
关键词
凡纳滨对虾
氯霉素
有益微生物
体液免疫因子
Litopenaeus vannamei
chloramphenicol
antibiotic
probiotic
humoral immune factor
