不同培养条件对坛紫菜叶状体体细胞发育分化影响的研究

Effects of different culture conditions on development and differentiation of somatic cells of Porphyra haitanensis thallus

本文以野生坛紫菜（Porphyra haitanensis）叶状体为材料，通过海螺酶酶解获得叶状体单离体细胞，探讨了细胞密度、光照强度对坛紫菜叶状体体细胞发育分化的影响．研究结果表明：细胞密度对坛紫菜叶状体单离体细胞的分裂、出苗具有明显的影响．其中，细胞培养3～5d时，分裂率随着细胞密度的增大而降低；5d时，1×10^3个／孔组的分裂速度显著大于其余各组的（P〈0．05）；12～19d时，随着细胞密度的增大，体细胞出苗率相应减小．光照强度对坛紫菜叶状体单离体细胞的分裂、出苗也具有显著影响．细胞培养5d时，分裂率随着光照强度的增大而增大；5～7d时，体细胞分裂速度随着光照强度增大而增大；9—19d培养期内，出苗率随着光照强度的增大而增大．本实验结果为坛紫菜转基因育种等研究提供了可资借鉴的基础资料．

Somatic cells of wild Porphyra haitanensis thallus are appropriate for the study of cell differentiation. The result shows that development and differentiation the ceils are affected significantly by culture conditions. It shows that division and regeneration of the ceils are affected significantly by ceil density. Divistion rate decreased with increasing ceil density in 3 - 5 days ; Division speed of 1 × 10^3 cells/well group wasis higher than those of other groups （p 〈0. 05） in 5 days;Regeneration rates of the cells decreased with increasing cell density in 12 - 19 days. It is also shown that divistion and regeneration of the cells are affected significantly by illumination intensity. Divistion rate is increased with increasing illumination intensity in 5 days ; Division speed increased with increasing the illumination intensity in 5 - 7 days;Regeneration rates of the cells increased with increasing illumination intensity in 9 - 19 days. Our data can be referenced in the study of transgenic breeding for Porphyra.