mRNA差异显示技术在植物学领域的应用

Advance in mRNA differential display reverse transcription PCR and its application in botany

概述了近年来在克隆差异表达基因方面应用比较广泛的mRNA差异显示技术的原理、优缺点及操作过程中应注意的事项。并对最近几年该技术在植物生长发育基因、逆境胁迫基因、雄性不育机理和杂交优势机理、激素调控应答基因等方面的研究现状做了综述,还提出了该技术在未来植物学研究上的应用前景。

mRNA differential display reverse transcription-PCR （DDRT-PCR） is a useful technique for analyzing differential gene expression at transcription level.The principles and characters of DDRT-PCR and some major procedures during its operation were introduced. Its advance in developmental gene, stress regulation gene, mechanism of male sterile and heterosis, hormone regulation and control gene in plants was reviewed. The application prospective of DDRT-PCR was put forward in the paper.