荧光定量PCR技术验证Sinofiler试剂盒的适宜模板量

Analysis of Appropriate Amount of Template DNA for Sinofiler Kit by Real Time Quantitative PCR Technique

目的探讨Sinofiler试剂盒适合扩增的模板DNA用量。方法选取经Sinofiler试剂盒基因分型且图谱完整、扩增均衡性好的DNA样本,进行荧光定量PCR检测。结果实验结果表明,在12.5μL体系中,1.29～1.51ng的模板DNA能够获得理想的分型结果。结论应用荧光定量PCR技术检测不同试剂盒适合扩增的模板DNA用量是一种准确、有效的方法。

Objective To explore the appropriate amount of template DNA for Sinofiler Kit. Methods The DNA samples with ideally genotyped results by Sinofiler Kit were detected by real-time quantitative PCR assay. Results It was shown that 1.29-1.51 ng of template DNA in 12.5 μL reaction volume was optimal for STR genotyping with Sinofiler Kit. Conclusion Real time quantitative PCR is an accurate and necessary technique for detection of appropriate amount of template DNA for different kits.