摘要
利用光镜和电镜研究了短期渗透胁迫下玉米(Zea mays L.)根冠区超微结构和ATP酶活性变化。经历了质壁分离后,在根冠细胞仍然可以观察到许多从“撤退”的周质出发向细胞壁辐射的纤丝。利用ATP酶活性产物(磷酸铅)在原生质膜和细胞壁处沉积的特点,发现这些纤丝是质膜围绕原生质而形成的管状结构;在质壁分离过程中,这些纤丝依然与胞间连丝相连。与对照(未胁迫)相比,受渗透胁迫细胞胞间连丝处ATP酶活性明显下降。能量代谢在局部区段的抑制会影响胞间连丝的生理活性,可能包括胞间连丝的扩散调节能力和分子扩散上限的改变。
With light and electron microscopy the substructural change and the ATPase activity of corn (Zea mays L.) root cap cells after short-term osmotic stress were studied. Some spoke-like fine strands originating from the departed periplasm and stretching towards cell wall could be observed even after plasmolysis. By observing the precipitation of ATPase activity product (lead phosphate) at plasma membrane and plasmodesmata, it was found that the fine strands were plasma membrane-lined channels surrounding the cytoplasm and that they still firmly connected to the plasmodesmata during plasmolysis. Compared with the control (unstressed), a sharp decrease of ATPase activity in the plasmodesmata of the stressed cells was observed. Inhibition of energy metabolism in these limited locales would affect the physiological activity, maybe including the regulation of permeability and the change of size exclusion limit (SEL) of plasmodesmata.
基金
Supported by the grants from the National Natural Science Foundation of China.
