姜黄素对大鼠小肠炎组织MPO、SOD作用的影响

Effects of curcumin on MPO and SOD of methotrexate-Induced small intestinal damage in rats

目的研究姜黄素对受损肠黏膜大鼠小肠组织髓过氧化物酶(MPO),超氧化物歧化酶(SOD)活性的影响,探讨姜黄素的抗炎机制。方法应用氨甲碟呤制备大鼠小肠炎模型,实验设正常对照组,模型对照组,柳氮磺胺吡啶(SASP,100mg/kg)组,姜黄素(100mg/kg)组。后2组每天灌胃给药1次,给药时间从造模后第2天开始至实验结束,共6d,第4天﹑第7天分别观察大鼠疾病活动指数(DAI)和结肠黏膜损伤指数(CMDI),光镜下组织学评分(HS),生化法检测大鼠小肠组织髓过氧化酶(MPO)及超氧化物歧化酶(SOD)活性。结果与正常组相比,模型组大鼠DAI、CMDI和HS明显增加(P<0.01),小肠组织MPO活性显著升高(P<0.01),SOD活性则显著下降(P<0.01),姜黄素可改善DAI,CMDI和HS(P<0.01),降低MPO活性(P<0.01),增加SOD活性(P<0.01)。结论姜黄素对大鼠小肠炎具有保护作用,其机制可能与减少脂质氧化及增加清除氧自由基的能力有关。

Objective To investigate the protective effect of curcumin on experimental rat enteritis and the possible mechanism. Methods Rat model of enteritis was induced by methotrexate （MTX） and sodium chloride. The experimental animals were randomly divided into four groups： normal group, model group, sulfasalazine （SASP） group and curcumin group. SASP （100 mg/kg）, curcumin （100 mg/kg）, and saline were administered to rats in corresponding groups by gastric irrigation daily for 6 days. The disease activity index （DAI）, colonic mucosal damage index （CMDI） and histological score （HS） of the rats were observed and evaluated. The level of myeloperoxidase（MPO） and superoxide dismutase（SOD） was measured by biochemical method. Results Compared with normal group, the DAd, CMDI and HS in model group was significantly increased, and the level of MPO was augmented .However, SOD activity was significantly reduced. Conclusion Curcumin has beneficial effect on rat enteritis by reducing lipid peroxidation and resisting oxygen free radicals.