固相萃取-高效液相色谱法测定水中痕量苯并(α)芘

Determination of Trace Benzo(a) Pyrene in Water by Solid Phase Extraction and High Performance Liquid Chromatography

目的建立水中痕量苯并(a)芘的检测方法。方法采用高效液相色谱法,以Waters PAH C18(250 mm×4.6 mm×5μm)为分析柱,甲醇为流动相,流速1.2 ml/min,荧光检测器检测,水样用ENVI-18柱富集并用四氢呋喃洗脱。结果方法最低检出限为0.002μg/L,线性相关系数r=0.999 9,平均回收率为86.4%,RSD=2.33%(n=6)。结论本方法可以对水中苯并(a)芘进行定量测定,且简便、准确、可靠。

Objective To develop a method to determine trace Benzo（a） pyrene in water. Methods The method of high performance liquid chromatography with fluorescence detection was adopted, a Waters PAH C18 （250 mm ×4. 6 mm× 5 mm） was used as analytic column and the flow rate of mobile phase, methanol was 1.2 ml/min. The analyte was enriched by ENVI - 18 cartridge and eluted with tetrahydrofuran. Results The detection limit of method was 0. 002 mg/L and the correlation coefficient was 0. 999 9. The average rate of recovery was 86.4% and RSD was 2. 33% （ n = 6 ） . Conclusion The method is simple, accurate and reliable for the determination of Benzo（a） pyrene in water.