重组大肠杆菌表达的枯草芽孢杆菌腺苷磷酸化酶的应用研究

The Application Research of Adenosine Phosphorylase from Bacillus sp. in E.Coli

利用枯草芽孢杆菌嘌呤核苷磷酸化酶基因deoD在大肠杆菌BL21(DE3)中表达所得到的蛋白为酶液,催化核苷、2′-脱氧核苷与嘌呤及其类似物之间的反应。结果表明,嘌呤类核苷与嘧啶类核苷相比,前者达到反应平衡的时间较短、产物的得率(或底物的转化率)较高;腺苷磷酸化酶对核苷类的催化活性要比对脱氧核苷类的催化活性强;就碱基而言,对嘌呤和嘌呤类似物的催化活性基本相同。

The application research of adenosine phosphorylase coded by deoD gene from Bacillus sp. in E. coli BL21 （DE3） was studied. The enzyme was used to catalyze the reaction between nucleosides（or 2＇-deoxynucleosides） and purines （or purine analogs）. The results showed that the reaction with purine nucleosides reached balance faster than that with pyrimidine nucleosides, with higher product yield （or substrate conversion rate）. This enzyme was more active to nucleosides than to deoxynucleosides, and its activity was roughly equal for purines and purine analogs in terms of bases.