皖北地区类风湿关节炎与HLA-DR4基因亚型的关联性研究

Relationship between HLA-DR4 Antigen Gene and Rheumatoid Arthritis in North of Anhui Province

目的研究人类白细胞抗原基因中的HLA-DR4基因亚型与皖北地区汉族人群类风湿关节炎(rheum atoid ar-thritis,RA)的发病及临床表现的关联性。方法采用特异性引物聚合酶链反应技术,检测皖北地区汉族人群102例RA患者及40例健康人HLA-DR4基因亚型,分析其与RA发病及临床表现的关联性。结果①对HLA-DRβ1基因亚型研究结果发现仅仅HLA-DRβ1*0405基因与RA有显著关联,2组比较差异有统计学意义(P<0.05),表明HLA-DRβ1*0405基因是皖北地区汉族人群RA易感基因的主要亚型。②HLA-DRβ1*0405阳性组晨僵时间明显比阴性组长(P<0.05),RF滴度阳性组明显比阴性组高,肺部病变阳性组比阴性组增多,差异有统计学意义(P<0.05)。结论皖北地区RA易感性与HLA-DRβ1*0405基因相关。HLA-DRβ1*0405基因与晨僵时间、RF有关,HLA-DRβ1*0405阳性患者更容易发生肺间质纤维化。

Objective To investigate the relationship between HLA-DR subtype and clinical teatures ot rheumatoid arthritis （RA） in Han population in north of Anhui. Methods Polymerase chain reaction-sequence specific primer（ PCR-SSP） was used to determine HLA-DR0405 gene subtype in 80 patients with RA and 40 healthy controls from Han popultion in north of Anhui. Results Susceptibility of RA was associated with HLA-DRβ1 ＊ 0405 subtype in Han population in north of Anhui （P 〈 0.05 ）. The time of ankylosis in the morning in RA patients with HLA-DRB1 ＊ 0405 subtype was longer than RA patients without it（ P 〈 0.05 ）. Conclusions The susceptibility of RA in Han population in north of Anhui is related to HLA-DRβ1 ＊ 0405 subtype.