肝细胞癌患者HCV感染及其致癌机理研究

The Study of HCV Infection in The Hepatocellular Carcinoma and its Carcinogenesis

目的：揭示肝细胞癌患者的ＨＣＶ感染情况及其致癌机理。方法：应用免疫组织化学ＳＰ法对４６例肝细胞癌癌组织及其３８例癌旁肝组织中丙型肝炎病毒核心抗原作了定位研究，并进一步检测其中癌基因ｒａｓ、ｃｍｙｃ和抑癌基因ｐ５３的蛋白表达。结果：核心抗原的检出率分别为２１７％和３６８％，４６例肝癌患者中１６例存在丙型肝炎病毒感染；ｐ２１、ｃｍｙｃ和ｐ５３蛋白在癌组织中的检出率分别为５８７％、６７４％和３０４％；丙型肝炎病毒感染的阳性与阴性组患者之间的ｐ２１、ｃｍｙｃ和ｐ５３蛋白的表达无显著性差异（Ｐ＞０．０５）；两组患者癌旁肝硬化的阳性率分别为８７５％和８６７％（Ｐ＞００５）。结论：上述结果提示丙型肝炎病毒致肝细胞癌的机理可能与ｒａｓ、ｃｍｙｃ和ｐ５３基因的激活或失活无关。

Aim:To elucidate the HCV infection in the hepatocellular carcinoma and its possible carcinogenesis.Method:The HCV core antigen、rasp 21 c myc and p53 proteins were studied by the immunohistochemical SP methed in the 46 cancerous tissues of HCC patients and 38 pericancerous tissues.Results:The positive rates of HCV core antigen were 21.7% and 36.8%,respectively,16 had infection of HCV in the 46 patients,The positive rates of p21、c myc and p53 proteins were 58.7%、67.4% and 30.4% in the cancerous tissues.No association was found between HCV infection and expression of p21、c myc and p53 proteins (P>0.05).Positive rates of cirrhosis in the peri cancerous tissues were 87.5% and 86.7% (P>0.05).Conclusion:It indicated from above findings the carcinogenesis of HCV wasnt associated with the activation of ras and c myc oncogenes and in activation of p53 gene.The further research was needed in this field.