利用荧光响应的台式生化需氧量传感仪器的研制

Development of Portable Sensing Apparatus for Biochemical Oxygen Demand Determination Based on Fluorescent Response

利用氧荧光猝灭原理研制了台式生化需氧量（BOD）传感检测仪器。仪器的工作系统由检测系统和软件系统两部分组成。该检测仪操作简便,检测快速,可进行海水BOD含量的现场检测。仪器的性能考察结果表明,在0.5~50mg/L浓度范围内BOD响应的线性相关系数为0.9978。实际海水样品的检测结果显示,仪器对海水样品的平行测定结果相对标准偏差小于±5%,并且与标准五日生化需氧量（BOD5）方法所获得的数值的相关性为0.99。仪器具有较长的使用寿命和良好的稳定性。

A portable biochemical oxygen demand（BOD） sensing apparatus based on the fluorescent quenching of oxygen was developed for BOD determination. The apparatus showed good characteristics such as easy manipulation, rapid response and practicable in situ measurement for BOD in seawater. The linear fluctuant coefficient （R2） in the BOD concentration range from 0.5 to 50 mg/L was 0.9978. A reproducible response of less 5 % for the BOD apparatus could be obtained in the analysis of real seawater samples. The values estimated by this biosensing apparatus correlated well with those determined by the conventional 5-day BOD （ BOD5 ） test. The investigation results indicated that the apparatus presented its long application life and good stability.