二苯乙烯苷对过度表达α-synuclein蛋白的转基因COS-7细胞株的影响

Effects of Tetrahydroxy-stilbene Glycoside on Over-expression of α-synuclein Protein in Transgenic COS-7 Cells

目的观察二苯乙烯苷（TSG）在体外试验中对COS-7细胞株过度表达α-synuclein蛋白的影响。方法将不同浓度的TSG与转染α-synuclein基因的COS-7细胞共孵育24h,应用二甲基噻唑蓝（MTT）法检测细胞存活率,免疫细胞化学和Western免疫印迹法检测α-synuclein蛋白的表达,观察TSG对其的影响。结果TSG在12.5～200.0μmol/L浓度范围内与COS-7细胞孵育24h不影响细胞存活率,而在免疫细胞化学和Western免疫印迹分析中均显示TSG剂量依赖性地抑制α-synuclein蛋白表达和聚集。结论TSG在体外对COS-7细胞过度表达α-synuclein有明显抑制作用。

Objective To explore the effects of 2,3,5,4′ tetrahydroxy stilbene-2 13 D-glycoside （TSG） on the over-expression and aggregation of α-synuelein in vitro. Methods TSG in different concentrations was incubated with α-synuelein transgenie COS-7 cells for 24 h. The cell viability was measured by MTT method. The expression of α-synuclein protein was determined by immunocyto- chemistry and Western blotting method. Results Incubation of TSG at the range of 12.5-200.0 μmol/L with α-synuclein transgenic COS-7 cells for 24 h did not influence cell viability, but a dose-dependently inhibition for the over-expression of α-synuclein protein could be observed in the tests of immunocytochemistry and Western blotting. Conclusion TSG can inhibit the over-expression of α-sy nuclein protein in COS-7 cells in vitro.