川芎嗪对顺铂耳中毒诱导型一氧化氮合酶表达的影响

Effect of Ligustrazine on Expression of Induced Nitricoxide Synthase with Cisplatin-induced Ototoxicosis

目的观察诱导型一氧化氮合酶(iNOS)在川芎嗪拮抗顺铂耳毒性作用中的表达情况。方法将50只健康白色红目豚鼠随机分为对照组、顺铂组和中药组,各组动物均于用药前及停药后测试听觉脑干诱发电位(ABR),采用耳蜗铺片观察毛细胞损伤情况,应用免疫组织化学方法测定各组动物耳蜗内iNOS的表达情况。结果ABR阈值对照组为(40.94±6.75)db,顺铂组为(76.26±4.54)db,中药组为(58.98±5.82)db,三组间的差异有非常显著性意义(P<0.01);对照组不表达iNOS,顺铂组Corti氏器和螺旋神经节细胞胞浆内iNOS强阳性表达,中药组iNOS阳性表达。结论川芎嗪可以通过抑制iNOS的表达对抗顺铂的耳蜗毒性。

Objective To observe the expression of induced nitricoxide synthase （iNOS） in ligustrazine against cisplatin-induced ototoxicity. Methods Fifty white guinea pigs with normal hearing were randomly divided into the control group, ligustrazine group and traditional Chinese drug （TCD） group. The alternations of hearing function were evaluated by the examination of auditory brainstem response （ABR）. Some specimens were perfused by AgNOa and observed the damage of bristle cell. And some were sent for immunohistochemistry to detect the expression of iNOS. Results Compared with the control group, the threshold of ABR in ligustrazine group and TCD group were significantly different. There was a significant difference between the ligustrazine group and TCD group. There was no expression of iNOS in Cortis＇, spiral ganglion cell and atria vascularis in normal cochlear （control group）. In the ligustrazine group, the expression became stronger in spiral ganglion cell, stria vascularis, spiral ligament and Cortis＇. INOS also expressed in TCD group. Conclusion Ligustrazine can antagonize cisplatin-induced ototoxicity by inhibiting the expression of iNOS.