轻度认知功能障碍患者淋巴细胞糖原合成酶激酶-3活性的变化

Change of glycogen synthase kinase-3 activity in lymphocytes in patients with mild cognition impairment

目的探讨轻度认知功能障碍(MCI)患者淋巴细胞糖原合成酶激酶-3(GSK-3)活性的变化。方法应用免疫印迹法检测不同病程(1年,2年,3年)MCI患者及正常老年人(正常对照组)外周血淋巴细胞GSK-3βSer9位点的磷酸化水平及总GSK-3β水平。结果1年,2年及3年MCI组淋巴细胞GSK-3βSer9位点磷酸化水平比正常对照组显著降低(均P<0.01);总GSK-3β水平与正常对照组相比差异无统计学意义;MCI组间淋巴细胞GSK-3βSer9位点磷酸化水平及总GSK-3β水平差异无统计学意义(均P>0.05)。结论MCI患者淋巴细胞GSK-3的活性升高,对MCI的诊断可能有参考价值。

Objective To explore the change of glycogen synthase kinase-3 （GSK-3） activity in lymphocytes in patients with mild cognition impairment （MCI）. Methods The phosphorylation of GSK-3β at Ser 9 site and total level of GSK-3β in peripheral blood were measured by Western-blot in normal control group, one-year MCI group, two-year MCI group and three-year MCI group. Results The phosphorylation of GSK-3β at Ser 9 site were lower in 1-year MCI group, 2-year MCI group and 3-year MCI group, compared with normal control group（ all P 〈0. 01 ）. There were not significant difference in total level of GSK-3β among MCI group and normal control group and in phosphorylation of GSK-3β at Ser 9 site and total level of GSK-3β in MCI group （ all P 〉 0. 05 ）. Conclusion The activity of GSK-3 in lymphocytes in MCI patients is raised, which implies a subsidiary value of MCI diagnosis.