氧化苦参碱增强环磷酰胺对小鼠Lewis肺癌生长的抑制作用

Oxymatrine promotes the antitumor activity of cyclophosphamide against Lewis lung carcinoma in mice

目的:观察氧化苦参碱及其联合环磷酰胺对小鼠Lewis肺癌生长的影响,探讨其可能的作用机制。方法:C57BL/6小鼠体内接种Lewis肺癌瘤细胞悬液制备移植瘤模型,氧化苦参碱(80、40、20 mg/kg)单用和联合环磷酰胺(30 mg/kg)灌胃给药后,计算平均抑瘤率,绘制肿瘤生长曲线,放射免疫法测定血清中表皮生长因子(EGF)含量,并与模型对照组作对比。结果:与模型对照组相比,单用氧化苦参碱抑瘤率没有差异,80、40、20 mg/kg氧化苦参碱联合环磷酰胺组肿瘤抑制率明显升高,分别为66.62%、61.70%、59.04%(P<0.01);高、中剂量氧化苦参碱联合环磷酰胺组血清EGF浓度明显低于模型对照组及环磷酰胺组(P<0.01)。结论:氧化苦参碱可以增强环磷酰胺对小鼠Lewis肺癌的生长抑制作用,可能与其降低血清EGF浓度有关。

Objective：To observe the anti-tumor activity of oxymatrine alone or in combination with cyclophosphamide against Lewis lung carcinoma in mice and to discuss the related mechanisms. Methods： Lewis lung carcinoma cells were trans planted subcutaneously to establish cancer transplantation model in mice. Animals were orally given oxymatrine（80, 40 and 20 mg/kg） alone or in combination with cyclophosphamide（30 mg/kg, gastric lavage）. The mean tumor inhibitory rate was calculated and the growth curve of tumors was plotted. Radio immunoassay（RIA） was used to determine the serum concentration of epidermal growth factor （EGF）. Comparison was made between the treatment groups and the control group. Results： The inhibitory rates were not significantly different between the control group and the pure oxymatrine treatment group. When combined with cyclophosphamide, the inhibitory rates of oxymatrine were significantly increased （P〈0.01）. When at 80, 40 and 20 mg/kg, oxymatrine inhibited tumor growth by 66.62%, 61.70% and 59.04%, respectively. Also, when combined with cy clophosphamide, oxymatrine at 40 and 80mg/kg significantly decreased EGF levels compared with those of model group and pure oxymatrine groups （P〈0.01）. Conclusion： Oxymatrine can enhance the inhibitory effect of cyclophosphamide against Lewis lung carcinoma, and the mechanism may be related to the decrease of serum EGF level.