摘要
目的:纯化培养和鉴定新生大鼠的脊髓星形胶质细胞,建立稳定的星形胶质细胞培养体系,为研究创伤后慢性神经病理性痛的脊髓机制奠定基础。方法:根据成纤维细胞与星形胶质细胞生长倍增时间的差异,用阿糖胞苷(Ara-C)抑制成纤维细胞生长分裂的方法获取星形胶质细胞,用胶质原纤维酸性蛋白(GFAP)免疫荧光法对其鉴定。同时与差速贴壁培养法进行对比。结果:用Ara-C处理后,可获得较高纯度的星形胶质细胞,经鉴定其纯度可达90%以上,而对照组几乎全部为成纤维细胞。结论:Ara-C处理能彻底清除成纤维细胞,从而保持星形胶质细胞的正常生长繁殖,达到纯化星形胶质细胞的目的。
Objiective: To find out the method for purifing astrocytes from the spinal cord of neonatal SD rats, and establish a pure cell culture system for the investigation of the effect of the spinal cord on chronic neuropathic pain development. Methods: According to the different population doubling time between astrocyte and fibroblast, cells were cultured with suppression of fibroblasts (Ara-c) and identified with immumofluorescence method against glial fibrillary acidic protein (GFAP). The adherent culture with different time was used as the control group. Results: In the Ara-C treated group, a pure culture of astrocyte cells was obtained. After being identified, the purity was over 90%. In contrast, there were almost full of fibroblasts in the control group. Conclusion: Ara-C can suppresses the proliferation of fibroblasts, and enable to maintain the normal growth of astrocyte cells.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2008年第2期207-209,共3页
Chinese Journal of Anatomy
基金
湖北省科技厅自然科学基金(2005ABA080)
湖北省卫生厅青年科技人才基金(QJX2005-16)
关键词
脊髓
星形胶质细胞
培养
纯化
鉴定
spinal cord
astrocyte
culture
purification
identification