摘要
目的:建立体外培养Schwann细胞的缺氧模型。方法:取体外培养Schwann细胞,置于通入体积分数为5%CO2和95%N2的有机玻璃盒中继续缺氧培养10、15和20min,用H-E染色观察细胞形态,MTT比色试验检测细胞的活力。结果:缺氧10min组细胞形态及活力与正常组无明显差异;而缺氧15min组的细胞突起回缩,活力为缺氧前的66·3%;缺氧20min组的细胞多数死亡,活力仅为缺氧前的20·6%。结论:本方法可以成功建立有效、简便、易行的Schwann细胞缺氧模型。
Objective: To establish a hypoxic model of culturing Schwann cells in vitro. Methods: Schwann cells harvested from 2-3d SD rat pups were incubated in a special chamber (5%CO2, 95%N2) for 10,15 and 20 minutes. The morphology and viability of cells were evaluated by HE stain, MTT activity assay respectively. Results: Compared with the control group, the cells in hypoxia condition for 10minutes showed no significant difference; furthermore, the processes of cells withdrew under hypoxia condition for 15minutes and their viabilities were 66.3%; the viabilities of 20-minute-hypoxia cells group were just 20.6%. Conclusion: The present method may make a successful model of hypoxic Schwann cells.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2008年第2期210-212,共3页
Chinese Journal of Anatomy
基金
上海市高等学校科学技术发展基金(No.03BK15)
上海市教委第四期重点学科资助(ZDXK2001)