体外培养Schwann细胞缺氧模型的建立

Establishment of hypoxic model of culturing Schwann cells in vitro

目的:建立体外培养Schwann细胞的缺氧模型。方法:取体外培养Schwann细胞,置于通入体积分数为5%CO2和95%N2的有机玻璃盒中继续缺氧培养10、15和20min,用H-E染色观察细胞形态,MTT比色试验检测细胞的活力。结果:缺氧10min组细胞形态及活力与正常组无明显差异;而缺氧15min组的细胞突起回缩,活力为缺氧前的66·3%;缺氧20min组的细胞多数死亡,活力仅为缺氧前的20·6%。结论:本方法可以成功建立有效、简便、易行的Schwann细胞缺氧模型。

Objective： To establish a hypoxic model of culturing Schwann cells in vitro. Methods： Schwann cells harvested from 2-3d SD rat pups were incubated in a special chamber （5%CO2, 95%N2） for 10,15 and 20 minutes. The morphology and viability of cells were evaluated by HE stain, MTT activity assay respectively. Results： Compared with the control group, the cells in hypoxia condition for 10minutes showed no significant difference; furthermore, the processes of cells withdrew under hypoxia condition for 15minutes and their viabilities were 66.3%; the viabilities of 20-minute-hypoxia cells group were just 20.6%. Conclusion： The present method may make a successful model of hypoxic Schwann cells.