摘要
目的探讨汉黄芩素对脂多糖(LPS)诱导的小胶质细胞激活时所分泌的促炎性因子的影响。方法采用LPS诱导的小胶质细胞活化模型,用ELISA法测定细胞外液所分泌的一氧化氮(NO)、肿瘤坏死因子-α(TNF-α)和白细胞介素-6(IL-6)的含量。结果LPS能激活小胶质细胞,并且能使其NO、TNF-α和IL-6的分泌量增加,汉黄芩素浓度为20-30μmol/L时能有效降低NO、TNF-α和IL-6的含量。结论汉黄芩素有抑制促炎性因子分泌的作用。
Objective To observe the effects of wogonin on lipopolysaccharide (LPS-induced) proinflammatory cytokine production in activated microglia. Methods By using LPS-induced microglia activation model, nitric oxide (NO), tumor necrosis factor-alpha (TNF- α ) and interleukin-6 (IL-6) secreted by extracellular fluid were measured by ELISA. Results Microglia markedly produced NO, TNF-α and IL-6 in response to LPS. Wogonin, administered at 20- 30 μmol/L, significantly reduced the production of NO, TNF-α and IL-6 in microglia. Conclusion Wogonin has an inhibitory effect on the production of proinflammatory cytokines.
出处
《西安交通大学学报(医学版)》
CAS
CSCD
北大核心
2008年第2期230-232,共3页
Journal of Xi’an Jiaotong University(Medical Sciences)
基金
留学回国人员科研启动基金资助项目(No.教外司留[2005]383号)
关键词
汉黄芩素
小胶质细胞
脂多糖
一氧化氮
肿瘤坏死因子-Α
白细胞介素-6
wogonim microglia
lipopolysaccharide (LPS)
nitric.oxide (NO)
tumor necrosis facter-alpha (TNF- α )
interleukin-6 (IL-6)