MODS大鼠外周血单个核细胞内核因子-κB和过氧化物酶体增殖物激活受体-γ的表达

Expression of nuclear factor-kappa B and peroxisome proliferators activated receptor-γ in peripheral blood monouclear cells of multiple organ dysfunction syndrome rats

目的探讨多器官功能障碍综合征(MODS)大鼠外周血单个核细胞(PBMC)内核因子-κB(NF-κB)和过氧化物酶体增殖物激活受体-γ(PPARγ)的表达及相互关系。方法健康雄性SD大鼠40只,随机分为四组(每组10只),正常对照组,脂多糖(LPS)刺激组,罗格列酮(ROSI)预处理组,选择性拮抗剂2-氯-5-硝基苯胺(GW9662)预处理组。免疫细胞化学法检测PBMC内PPARγ、NF-κBp65的表达活性,并进行图像分析。结果(1)在正常组大鼠PBMC内NF-κBp65、PPARγ表达均较少。LPS刺激组NF-κBp65表达与正常组比较显著增加(P<0.01);PPARγ表达稍有增高,与正常组比较差异无统计学意义(P>0.05)。ROSI组NF-κBp65表达与LPS刺激组比较显著降低(P<0.01);PPARγ表达与LPS刺激组比较显著增加(P<0.01)。GW9662组NF-κBp65表达与LPS刺激组比较差异无统计学意义(P>0.05);PPARγ表达显著降低,与正常组比较差异无统计学意义(P>0.05)。(2)相关分析结果表明,PBMC内NF-κB和PPARγ活性变化在LPS刺激组、ROSI组、GW9662组呈显著负相关(LPS刺激组:r=-0.916,P<0.01;ROSI组:r=-0.605,P<0.05;GW9662组:r=-0.961,P<0.01)。在正常对照组无明显相关性(r=0.185,P>0.05)。结论PPARγ可能是通过抑制NF-κB的活性而对MODS大鼠起保护作用。

Objective To study the expression of nuclear factor-kappa B （NF-κB） and peroxisome proliferators activated receptor-γ（PPARγ） in peripheral blood monouclear cells （PBMC） of multiple organ dysfunction syndrome rats. Methods Forty male SD rats were randomly divided into 4 groups （ n = 6 per group） ：vehicle control group, endotoxin （LPS） group, rosiglitazone （ROSI） pretreatment group, and PPAR-γ antagonist GW9662 pretreatment group. To detect the expression of NF-κB and PPARγ, immunocytochemical method and image analysis were carried out. Results The expression of NF-κB p65 and PPAR-γ were low in vehicle control group. In LPS group the expression of NF-κB p65 was significantly higher than in vehicle control group （ P 〈 0. 01 ） ; the expression of PPARγ had no significant difference compared with vehicle control group （ P 〉 0. 05 ）. In ROSI group the expression of NF-κB p65 was significantly lower than in LPS group （P 〈 0. 01 ） ;the expression of PPARγ was significantly higher than in LPS group （ P 〈 0. 01 ）. In GW9662 group the expression of NF-κB p65 had no significant difference compared with LPS group（ P 〉 0.05 ） ;the expression of PPARγ had no significant difference compared to vehicle control group（ P 〉 0. 05 ）. The study revealed a significant negative correlation between NF-κB and PPARγ in LPS group, ROSI group and GW9662 group. There was no significant correlation in vehicle control group. Conclusions PPARγ maybe protect the MODS rats by repressing the expression of NF-κB.