摘要
为了满足市场对单面针Zanthoxylum dissitum Hemsl资源的需求,解决单面针自然繁殖率低的问题,采用L9(34)正交设计,以单面针种子胚为外植体进行胚培养.结果表明:6-BA、NAA、消毒时间3因素的最佳水平组合为MS+6-BA 1 mg/L+NAA 0.4mg/L+消毒时间8 min,发芽率可达86.7%.
In order to satisfy the market＇s demands for the resources of Zanthoxylum dissiturn Hemsl, this paper conducted some research on the problem of its natural propagation, cooperating with Zhuzhou Qianjin Pharmaceutical Co. Ltd. Using its embryos as in vitro culture material, orthogonal design L9 （34） as the test method, and setting up 6-BA, NAA and disinfected time as the three main factors （every factor having 3 levels）. The results indicate that the best level combination is： MS4-6-BA 1mg/L＋NAA 0. 4 mg/L＋ 8 min of disinfected time, the germination rate being 86.7%.
出处
《中南林业科技大学学报》
CAS
CSCD
北大核心
2008年第1期130-133,共4页
Journal of Central South University of Forestry & Technology
基金
湖南省自然科学基金项目“药用植物单面针的生物学特性研究(07JJ5041)”
株洲市重点科技项目“药用植物单面针组培技术中试研究及组培苗栽培技术示范”
株洲千金药业股份公司合作项目“单面针人工快繁技术中试研究”
关键词
生物技术
单面针
胚
正交设计
组织培养
发芽率
bio-technology
Zanthoxylum dissitum Hemsl
embryo
orthogonal design
tissue culture
germination percentage
