论著p53基因突变与非霍奇金淋巴瘤病因学关系研究

Study on p53 gene mutations in non-Hodgkin's lymphomas

目的:研究p53基因突变在非霍奇金淋巴瘤(NHL)发生发展中的作用。方法:在37例非霍奇金淋巴瘤组织中采用PCR-单链构象多态性分析法(PCR-SSCP)进行p53基因外显子5～8的突变检测。结果:p53基因总突变率为54.1%,惰性组、侵袭性组、高侵袭性组突变率分别为0.0%、58.1%、100.0%;T和NK细胞组和B细胞组突变率分别为37.5%、58.6%。结论:非霍奇金淋巴瘤中存在p53基因的突变,p53基因的突变在NHL的发展演变中可能起重要作用。

Objective：To evaluate the mutation of p53 gene in pathogenesis of non-Hodgkin＇s lymphoma. Methods：Thirty-seven cases of non-Hodgkin＇s lymphoma were studied for frequency of p53 gene mutation in exon 5 to 8 with PCR-SSCP method. Results：In 37 cases of non-Hodgkin＇s lymphoma ,the frequency of p53 genie mutation was 54.1%. The the frequency of p53 genie mutation was detected in indolent（0.0%）,aggressive（58.6%） and highly aggressive group（100.0%）. The incidence of p53 genie mutation in T/NK cell lymphoma and B cell lymphoma was 37.5% and 58.6%, respectively. Conclusion：The mutation of p53 gene is present in NHL. Mutation of p53 gene might play a key role in pathogenesis and progression of lymphoma.