重症胰腺炎二十碳烯酸的异常代谢与大黄素、施他宁的作用

Effects of emodin and stilamin on abnormal metabolism of eicosanoids in acute haemorrhagic-necrotizing pancreatitis in rats

目的：探讨重症胰腺炎（AHNP）胰组织出血、坏死与二十碳烯酸类的异常代谢关系。方法：以牛黄胆酸钠诱发大鼠AHNP模型，测定前列腺素E2（PGE2）、6-酮-前列腺素F1a（6－keto－PGE1a）、血栓烷B2（TXB2）等变化，并以大黄素、施他宁药物干预，以了解AHNP时二十碳烯酸类的异常代谢和上述药物的作用。结果：重症胰腺炎时血浆TXB2显著增高．发病6小时达假手术组的4．5倍，而6－keto－PGF1a或PGE2的测定值则呈降低趋势．应用大黄素或施他宁后，TXB2测定值显著降低，施他宁组TXB2测定值较之于大黄素组降低更为显著；6－keto－PGE1a和PGE2则呈上升趋势．给药两组12小时生存率高于非治疗组；给药两组胰腺细胞坏死等病理损害减轻．结论：大黄素和施他宁对AHNP时TXB2等异常代谢有明显调整作用，与此相关的改善微循环和细胞保护机制可能是两药治疗AHNP的重要药理基础；联合应用大黄素与施他宁可能会有协同作用．

Objective: To study the effects of emodin and stilamin on abnormal metabolism in acute haemorrhagic-necrotizing pancreatitis in rats. Methods: In sodium taurocholate-induced AHNP in Sprague-Dawley rats, the plasma levels of prostaglandin E2 (PGE2),6-keto-prostaglandin F1a (6-keto-PGF1a) and thromboxane B2(TXB2) and the effects of emodin stilamin on them were investigated. Results: There was a significant increase of plasma TXB2 with a level of 4.5 times that in the sham-operated group 6 hours after triggering of the disease while 6-keto-PGF1a or PGE2 tended to decrease. However, TXB2 was significantly reduced either in emodin or stilamin group bu 6-keto-PGE1a or PGE2 tended to increase. Both emodin and stilamin gruops showed higher 12-hour survival rates than did the untreated group in which pathologic scoring under the microscope and ultrastructural obesrvation with transmission electron microscope revealed more severe acini necrosis. Conclusion: The benefit of emodin and stilamin in the treatment of AHNP can be ascribed to the modulation of abnormal synthesis of eicosanoids which improves pancreatic microcirculation and cytoprotection, and the combined use of the two drugs may be synergetic.