化疗对神经母细胞瘤MYCN基因表达及其影响

The Expression of MYCN Gene of Neuroblastoma After Chemotherapy and Its Effecting Factors

目的:观察 MYCN(N-myc)基因在神经母细胞瘤中的扩增及定位情况,探讨化疗对其表达的影响。方法:17例神经母细胞瘤病例,按 Evans 临床分期,其中6例术前接受化疗。用MYCN 基因探针进行核酸原位杂交。MIAS-300图像分析系统对切片进行灰度扫描并作 t′检验。结果:17例神经母细胞瘤标本中 MYCN 基因原位杂交检测阳性者11例(64.7%)。MYCN 基因定位于神经母细胞瘤细胞核内。临床分期对 MYCN 基因的表达无明显影响,而化疗对 MYCN 基因表达的影响作用明显。MYCN 检测阳性与阴性组的术前 VMA 测定值也有显著性差异。结论:MYCN 基因扩增反映神经母细胞瘤的疾病进程。化疗可抑制肿瘤细胞 MYCN 等胚胎性基因扩增,结合其临床 VMA 值的减低,表明肿瘤细胞的恶性行为受到抑制。

Objective:To observe MYCN gene expansion and location in neuroblastoma,to discuss the effective factors of MYCN gene expression.Methods:Seventeen cases of neuroblas- toma were classified by Evans Staging.Six of them received preoperative chemotherapy.In-situ hybridization was done by MYCN gene probe.The gray scale displaying of the sections was ana- lyzed by MIAS-300 image analysis system.Results:Eleven of 17 cases of neuroblastoma were positive to MYCN gene probe(64.7%).MYCN gene existed in the nucleus of the tumor cell. The clinic staging did not affect the MYCN gene expression,but the chemotherapy did markedly. The urine VMA between the MYCN positive group and negative group was also different preoper- atively.Conclusions:The expression of MYCN gene shows the progress of neuroblastoma. Chemotherapy restrains the expansion of embryonic genes of the tumor cells such as MYCN.The reduction of urine VMA shows that the malignant behavior of the tumor cell has been restrained.