神经母细胞瘤细胞增殖活性检测及意义

Detection of Proliferation Activity in Neuroblastomas and Its Clinical Significance

目的:探讨神经母细胞瘤(NB)细胞增殖活性在 NB 恶性程度和预后判断中的价值。方法:应用免疫组化法对20例 NB 标本中增殖细胞核抗原(PCNA)表达进行检测。结果:PCNA 表达指数(PEI)与肿瘤重量、有丝分裂核碎裂指数(MKI)呈正相关,r 分别为0.664和0.903;与术后生存时间呈负相关,r=-0.919;年龄<1岁、临床早期(Ⅰ、Ⅱ期)及ⅣS期、神经节神经母细胞瘤(GNB)、Shimada 病理分型 F 型及肿瘤切除者,其 PEI 均低于相应组别,P 值分别为>0.05、<0.05、<0.05、<0.01、<0.05。结论:PCNA 的表达与 NB 的恶性增殖、生长及浸润密切相关,是 NB 细胞增殖活性增高的分子标志;PEI 对判断 NB 的恶性程度和预后具有重要价值。

Objective:To investigate proliferating activity that may help understand the bio- logic behavior and prognosis of neuroblastoma(NB).Methods;Proliferating cell nuclear antigen (PCNA)of 20 specimens of NB were examined by immunohistochemistry stain.Results:There were positive correlation among PCNA expression index(PEI)and tumor's weight,and mitotic karyorrhexis index(MKI.r=0.664,0.903)and negative correlation between PEI and timing of survival(r=-0.919)。In cases under 1 years of age with GNB in early staging,total resection and favorable histopathologic type,the PEIs were significantly lower(P>0.05,<0.05,<0.05,< 0.01,<0.05,respectively).Conclusions:PCNA is a molecular marker of proliferating activity in NB.PEI may be useful to evaluate the biologic behavior and prognosis of NB.