葡萄膜炎患者外周血单个核细胞IRF1mRNA的表达及意义

Expression of IRF1 mRNA in peripherl blood mononuclear cells of uveitis

目的观察葡萄膜炎患者外周血单个核细胞(PBMC)IRF1mRNA的表达。方法收集42例葡萄膜炎患者(A组)和30例正常人(B组)外周血,采用淋巴细胞分离液密度梯度离心法分离外周血单个核细胞(PB-MCs),逆转录聚合酶链反应(RT-PCR)检测PBMCTh1细胞转录因子IRF1mRNA表达的相对含量。酶联免疫吸附法(ELISA)检测PBMCs培养上清液干扰素-γ(IFN-γ)表达水平。结果A组PBMC中IRF1mRNA相对含量显著高于B组(P<0.01),A组IFN-γ水平显著高于B组(P<0.01)。A组IRF1mRNA表达相对含量与IFN-γ的表达水平呈正相关(r=0.56,P<0.01)结论葡萄膜炎患者的Th1细胞过度分化可能与IRF1表达增强有关。

Objective To investigate the expression of IRF1 mRNA of peripheral blood mononuclear cells （ PBMCs ） in patients with Uveitis. Methods Peripheral blood was collected from 42 uveitis patients （ group A） and 30 healthy individuals （ group B ）. The PBMCs were isolated by Ficoll-Hypaque centrifugation method. The mRNA of IRF1 was determined by RT-PCR. Interferon-γ（IFN-γ） levels in the supematant were detected by enzyme linked immtmosorbent assay （ELISA） . Results IRF1 mRNA and IFN-γ were significantly upregulated in group A compared to group B （P 〈0. 01 ）. Positive correlations were found between the level of IRFI mRNA and IFN-γin groups A （ r = 0. 56, P 〈 0.01 ） . Conclusion Overdifferentiation of Thl cell may be related with expression of IRF1 in uveitis.