Lewis肺癌小鼠Th_1/Th_2免疫应答状态的观察

Immune response of Th_1/Th_2 in C57BL/6 mice with Lewis lung cancer

目的研究Lewis肺癌小鼠Th1/Th2免疫应答状态及其意义。方法C57BL/6小鼠16只按随机数字法分为肺癌模型组(A组)与正常对照组(B组),每组8只。A组采用Lewis肺癌单细胞悬液接种于C57BL/6小鼠右腋部皮下建立肺癌模型;B组则正常饲养未予处理。两组实验动物于实验末取血,应用酶联免疫吸附法(ELISA)检测血清中Th1细胞因子IFN-γ、Th2细胞因子IL-4浓度,并进行组间相关比较。结果A组小鼠血清中Th1相关细胞因子IFN-浓度明显低于B组小鼠,A组小鼠Th2相关细胞因子IL-4浓度明显高于B组小鼠,差异均有统计学意义(P<0.05);而FN-γ/IL-4比值A组小鼠相应低于B组小鼠,差异亦有统计学意义(P<0.05)。结论Lewis肺癌小鼠Th1/Th2免疫应答状态呈现明显Th1向Th2漂移,使肺癌细胞逃避机体免疫监视,并削弱机体的细胞免疫功能,这应是肿瘤发生发展的主要原因之一。

Objective To investigate the immune response of Th1/Th2 in C57 BL/6 mice with Lewis lung cancer and its significance. Methods 16 C57BL/6 mice were divided randomly into two equal groups： group A （Lewis lung cancer model, n ：8）; group B （normal control group, n=8）. The mice of group A received subcutaneous implant of the cultured Lewis lung cancer cells, but those of group B received no treatment. The Th1/Th2 cytokines in the serum of both groups were detected by EIISA and the related data were comparaed. Results Statictically, the concentration of IFN-γ in group A was lower than that in group B （P〈0. 05）, the concentration of IL-4 in group A was higher than that in group B （P〈0. 05）, and the IFN-γ/IL-4 ratio in group A was lower than that in group B （P〈0.05）. Conclusion The immune response of Th1/Th2 in C57 BL/6 mice with Lewis lung cancer showed clearly Th1 to Th2 drift, and that may account for the fact that the lung tumor cells escape the immune surveillance of the host and the fact that the body＇s immune cell function is weakened. This must have been one of the main reasons of tumorigenes.