兔出血症-巴氏杆菌病二联蜂胶灭活苗的研制及免疫效果的观察

Observation of Immunization Effect and development of Bicombinant Inactivated Vaccine of Rabbit Heamorrhagic Disease and Pasteurellosis

采用对本地区具有良好免疫原性的兔病毒性出血症强毒株和巴氏杆菌菌株,研制出常规二联灭活苗和蜂胶二联灭活苗。用血清学方法对常规二联灭活苗和蜂胶二联灭活苗免疫后兔病毒性出血症抗体的检测。结果表明:常规二联灭活苗免疫后7 d HI抗体可达5.0log2,15 d明显上升,达5.5log2,30 d达到高峰,可达8.0log2,;而蜂胶二联灭活苗在免疫后7 d HI抗体可达5.0log,15d明显上升,达5.5log2以上,20 d显著上升9.25log2以上,30 d达到高峰,可达11.0log2。说明蜂胶提高抗体效价作用明显,可显著增强免疫效果。

It was suitable for this region that Rabbit hemorrhagic disease virulent strain and pasteurellosis strain were good immunogenicity, bicombinant propolis inactivated vaccine and bicombinant inactivated caccine were developed successfully. To supervise the level of RHDV through serological method, the result showed that the titers of bicombinant inactivated caccine and bicombinant propois inactivated vaccine were 5.0 log2,5.51og2,5.61og2,6. 251og2,8.0log2 and 5.0log2,5.51og2,5.61og2, 9. 251og2,11.0log2 after 7 d , 10 d, 15 d, 20 d,30 d of injection respectively. Propois could produce could obviously improve antibody titer and enhange immune effect.