喷他脒的电化学行为及其应用

Electrochemical Behavior of Pentamidine and Its Application

采用循环伏安法研究了喷他脒在玻碳电极上的电化学行为,应用微分脉冲伏安法提出了喷他脒不需分离的直接测定方法。在0.1 mol·L^-1（pH 8.5）磷酸二氢钠-磷酸氢二钠溶液中,喷他脒于电位-1.56 V（vs.SCE）有一灵敏的还原波;试验证明该波是喷他脒的催化氢波,还原波峰电流与喷他脒浓度在2.0×10^-5-8.0×10^-4mol·L^-1范围内呈线性关系,相关系数为0.997 3,检出限为1.6×10^-5mol·L^-1;采用标准加入法测定尿液中样品,回收率在95.1%-103.8%之间。

Electrochemical behavior of pentamidine （PTM） was studied by cyclic vohammetry., As shown by experimental results, in a phosphate buffer of pH 8.5, a sensitive reduction wave produced by PTM was observed at the potential -1.56 V （vs. SCE）, which is proved to be a catalytic hydrogen wave. Linear relationship was held between peak current of the reduction wave and concentration of PTM in the range of 2. 0× 10^-5 -8.0× 10^-4mol · L^-1（r=0. 997 3）. Detection limit of the method was found to be 1.6× 10^-5mol · L^-1. Based on these facts, a derivative pulse vohammetric method of direct determination of PTM without necessity of separation was proposed. Recovery test was performed by addition of standard PTM solution to a urine sample, and the results obtained were in the range of 95. 1%-103. 8%.