金边瑞香茎尖脱毒及快繁技术

In Vitro Propagation of Virus-free by Soot-tip of Daphne Odora var.naginata Mak

报道了金边瑞香顶芽在MS＋0，05mg／L6-BA＋0．1mg／LNAA培养基中预培养20d，再在34℃（11h）＋23℃（13h）的昼夜温度交替下处理30d后，剥离0．2-0．3mm大小的茎尖在MS＋0，1mg／L6-BA＋0．1mg／LNAA培养基中的诱导成活率为80．0％，并获得脱毒苗。继代和增殖培养基为MS＋0．5-1．0mg／L6-BA＋0．1mg／LNAA，增殖系数4，1。试管苗在适宜培养条件下平均生根率为82．6％，在珍珠岩：泥炭=2：1基质中生根苗移栽成活率达到96．46％。

The following experimental results have been obtained：the terminal buds of Daphne odora var. naginata Mak were fires cultured on MS ＋ 0.05 mg/L 6 - BA ＋ 0.1 mg/L NAA for 20 days then in the condition of day and night with 34 ℃ （11 h） ＋ 23 ℃ （13 h） For 30 days. The surviral radio with shoot - tip of 0.2 -0.3 mm was 80.0% on MS ＋0.1 mg/L6 - BA ＋0. lmgLNAA . The virus -free seedlings were obtained. The terminal buds diffevertiated a numbers of young buds on MS medium containing 6 - BA0.5 - 1.0 mg/L and NAA 0.1 mg/L. On this medium shoot multiplication occurred at a 4.1 - fold rate. The average ratio of rooting of the high - quali tyseeding was 82.6% in the suitable condition of culture. The survival ratio of transplanting reached 96.5% in the medium （ pearlite ： peaty soil = 2 ： 1 ）.