蛛网膜下腔移植异体嗜铬细胞治疗神经性疼痛的实验研究

Subarachnoid adrenal medullary transplants reduce neuropathic pain in rats

目的:探讨蛛网膜下腔移植异体嗜铬细胞的抗伤害作用及作用特点。方法:按照Bennet所述方法将大鼠制作成压迫性坐骨神经痛模型。另取同系同代大鼠双肾上腺髓质,将其以髓质块或游离细胞形式植入模型鼠蛛网膜下腔。观察移植术后10周内模型鼠热、电痛阈及自发痛行为的变化,并观察纳络酮、酚妥拉明及芬太尼对动物痛反应的影响。结果:蛛网膜下腔移植异体髓质块或游离嗜铬细胞均能显著提高动物抗热、电痛效应,以及消除动物的自发痛行为。并能明显增强动物对芬太尼的敏感性,该作用能被纳络酮及酚妥拉明所逆转。结论:蛛网膜下腔移植嗜铬细胞能有效缓解神经性疼痛,并能增强宿主对外源性阿片制剂的敏感性。

Objective:To investigate the efficacy and characteristics of subarachnoid xenogenetic adrenal medullary transplants on neuropathic pain. Method: Eighty rats were assigned to 4 different groups before their right sciatic nerves were tied according to the method described by Bennet. Each rat received subarachnoidly xenogenetic medullary pieces or isolated chromaffin cells according to the groups. The basal pain thresholds to thermal and electrical stimuli were determined before nerve ligations,and the analgesiometric tests were repeated at weekly intervals following transplantation for 10 weeks. Behavioral indications of spontaneous pain were recorded concomitantly. The effects of naloxone, phentolamine and fentanyl on the antinociception of chromaffin cells were evaluated. Result:The pain thresholds to noxious thermal and electrical stimuli increased after transplantation of medullary pieces or isolated chromaffin cells subarachnoidly. The Behavioral indications of spontaneous pain and hyperalgesia to thermal and electrial were also eliminated after the transplantation. These antinociceptive effects can be reversed by naloxone and phentolamine. Conclusion:Transplanting xenogenetic chromaffin cells into subarachnoid space can reduce neuropathic pain effectively,and this antinociception is conducted via adrenoreceptors and opiate receptors.