麦冬多糖单次静脉注射在大鼠体内组织分布

Distribution of Polysaccharide MDG-1 after Single Intravenous Dose in Rats

目的建立柱前荧光衍生-高效凝胶色谱(HPGPC)法测定大鼠组织样品中麦冬多糖的方法,并研究麦冬多糖在大鼠体内的分布特点和规律。方法将SD大鼠单次静脉注射麦冬多糖50mg·kg-1后,于5,10,30,60min取各组织,用HPGPC法测定药物浓度。结果大鼠心、肝、脾、肺、胃、肾、脑的AUC分别为3.270,2.031,2.303,6.178,4.963,214.571,0.613μg·h·mL-1。结论绝大多数药物聚集在肾脏,并以尿的形式排泄,其他组织中含药量分布顺序从高到低依次为肺、胃、心、脾、肝、脑。

Objective To establish a method for measuring polysaccharide MDG-1 from tissues by pre-column FITC labeled-HPGPC and investigating the distribution of it in rat tissues. Methods Various tissues were obtained at 5,10,30 and 60 min, respectively, after a single intravenous administration of 50 mg · kg^-1 polysaccharide MDG-1. The concentration of MDG- 1 in samples were subsequently determined by HPGPC. Results Most of MDG-1 was readily accumulated in kidneys and excreted in urine. The distribution sequence of different tissues ranking from high to low was lung, stomach, heart, spleen, liver, brain. Conclusion MDG-1 could exert anti-myocardial ischemia effects directly on the target organ.